Introduction to Wastewater Based Epidemiology

Wastewater based epidemiology

Wastewater-based epidemiology (WBE) is a monitoring approach increasingly being used to assess trends in population chemical consumption, use and exposure as well as a surveillance tool from SARS-CoV-2 during the current Covid-19 pandemic. Here are a series of short video presentations and videos presenting the different components of WBE for both chemical and biological agents.

Wastewater-based epidemiology (WBE) explained. Toilet flushes from the community served by a catchment (Autosampler at WWTP) or sub-catchment (Passive sampler) captured over 24h. These samples are analysed for biomarkers of interest (e.g. SARS-CoV-2 RNA or an illicit drug metabolite) depending on the type of investigation. It is possible to calculate the amount of a biomarker excreted by the catchment population and knowing the catchment’s population allows this to be normalised to the number of people in each catchment. For some exogenous biomarkers it is possible to calculate the amount of the substance consumed. All is explained in the short-talks and videos below.

Videos

Please note, these videos were developed in 2021.

TitlePresenterDescriptionLink & Password
History of WBE internationally and in Australia - general introductions to WBEProf Kevin Thomas (QAEHS)An introduction to WBE and its historical development in Australia and internationally.Available here
How to Samples seriesJosh Tynan (QAEHS)A series of short videos showing how to sample.Available here
Sampling uncertaintiesMichael Lawrence (Bligh Tanner)An overview of sampling uncertainties and their importance in WBE.Available here
In sewer stabilityDr Jiaying Li (QAEHS)In-sewer stability of WBE biomarkers.Available here
Excretion factorsDr Phong Thai (QAEHS)The role of excretion factors for chemical biomarkers in calculating consumption.Available here
Archiving stabilityDr Jake O'Brien (QAEHS)The stability of chemical biomarkers under various storage conditions.Available here
Festivals, mining and small populationsA/Prof Cobus Gerber (UniSA)Application of WBE in small populations.Available here
National Wastewater Drug Monitoring ProgramProf Jochen Mueller (QAEHS)An overview of the Australian Criminal Intelligence Commission National Wastewater Drug Monitoring Program.Available here
SewersipperRory Verhagen (QAEHS)An introduction to the Sewersipper sampler.Available here
COVID MethodA/Prof Jianhua Guo (QAEHS)Methods to detect SARS-CoV-2 in wastewater.Available here
New psychoactive substancesDr Richard Bade (QAEHS)Detecting the use of new psychoactive substances in Australia.Available here
SewAus wastewater programDr Jake O'Brien (QAEHS)An introduction to the SewAus wastewater program.Available here

Surveillance of SARS-CoV-2 RNA in wastewater

Dr Warish Ahmed (CSIRO)Analysis of SARS-CoV-2 RNA fragments in Qld wastewaters.Link to video (YouTube)
Passive samplingA/Prof David McCarthyUnderstand how passive samplers help with near source tracking of COVID-19.Available here
End user perspective - ACICKatie Willis, Shane Nielson, Amber Migus (ACIC)

A Federal Government agency’s perspective on the utility of wastewater-based epidemiology for government.

Available here
End user perspective - COVIDDr Greg Jackson (Qld Health)

A State Government agency’s perspective on the utility of wastewater-based epidemiology for government

Available here